Package: rtpcr 2.2.0

Ghader Mirzaghaderi

rtpcr: qPCR Data Analysis

Tools for qPCR data analysis using Delta Ct and Delta Delta Ct methods, including t-test, Wilcoxon-test, ANOVA models, and publication-ready visualizations. The package supports multiple target, and multiple reference genes, and uses a calculation framework adopted from Ganger et al. (2017) <doi:10.1186/s12859-017-1949-5> and Taylor et al. (2019) <doi:10.1016/j.tibtech.2018.12.002>, covering both the Livak and Pfaffl methods.

Authors:Ghader Mirzaghaderi [aut, cre, cph]

rtpcr_2.2.0.tar.gz
rtpcr_2.2.0.zip(r-4.7-any)rtpcr_2.2.0.zip(r-4.6-any)rtpcr_2.2.0.zip(r-4.5-any)
rtpcr_2.2.0.tgz(r-4.6-any)rtpcr_2.2.0.tgz(r-4.5-any)
rtpcr_2.2.0.tar.gz(r-4.7-any)rtpcr_2.2.0.tar.gz(r-4.6-any)
rtpcr_2.2.0.tgz(r-4.6-emscripten)
manual.pdf |manual.html✨
DESCRIPTION |NEWS
card.svg |card.png
rtpcr/json (API)

# Install 'rtpcr' in R:
install.packages('rtpcr', repos = c('https://mirzaghaderi.r-universe.dev', 'https://cloud.r-project.org'))

Bug tracker:https://github.com/mirzaghaderi/rtpcr/issues

Pkgdown/docs site:https://mirzaghaderi.github.io

Datasets:

On CRAN:

Conda:

data-analysisqpcr

5.78 score 2 stars 15 scripts 504 downloads 11 exports 55 dependencies

Last updated from:6eb705234c. Checks:9 OK. Indexed: yes.

TargetResultTimeFilesSyslog
linux-develOK201
source / vignettesOK178
linux-releaseOK177
macos-releaseOK127
macos-oldrelOK148
windows-develOK124
windows-releaseOK124
windows-oldrelOK117
wasm-releaseOK134

Exports:ANOVA_DCtANOVA_DDCtcompute_wDCtefficiencylong_to_wideMeans_DDCtmeanTechmultiplotplotFactorTTEST_DDCtWILCOX_DDCt

Dependencies:bootclicodetoolscpp11dplyremmeansestimabilityfarvergenericsggplot2gluegtableisobandlabelinglatticelifecyclelme4lmerTestmagrittrMASSMatrixminqamultcompmvtnormnlmenloptrnumDerivpillarpkgconfigplyrpurrrR6rbibutilsRColorBrewerRcppRcppEigenRdpackreformulasreshape2rlangS7sandwichscalesstringistringrsurvivalTH.datatibbletidyrtidyselectutf8vctrsviridisLitewithrzoo

Manual
Overview | Functions | Quick start | Installing and loading | Input data structure | NOTE 1 | NOTE 2 | NOTE 3 | NOTE 4 | Handling missing data | Data Analysis | Amplification Efficiency | Relative expression | NOTE | Examples | Output | Data output | Plot output | Plot output: example 1 | How to edit output plots? | Plot output: example 2 | Plot output: example 3 | Post-hoc analysis | Checking normality of residuals | Mean of technical replicates | Running the shiny version of the rtpcr offline | Contact | Citation | References
Rendered fromManual.Rmdusingknitr::rmarkdown

Last update: 2026-09-20
Started: 2026-01-08

Calculation method
Calculations | A generalized calculation method | $$w\Delta C_t =\log_2(E_ | $$w\Delta C_t =\log_ | $$\text | References
Rendered frommethod.Rmdusingknitr::rmarkdown

Last update: 2026-06-19
Started: 2025-12-31