Package: rtpcr 2.1.9

Ghader Mirzaghaderi

rtpcr: qPCR Data Analysis

Tools for qPCR data analysis using Delta Ct and Delta Delta Ct methods, including t-test, Wilcoxon-test, ANOVA models, and publication-ready visualizations. The package supports multiple target, and multiple reference genes, and uses a calculation framework adopted from Ganger et al. (2017) <doi:10.1186/s12859-017-1949-5> and Taylor et al. (2019) <doi:10.1016/j.tibtech.2018.12.002>, covering both the Livak and Pfaffl methods.

Authors:Ghader Mirzaghaderi [aut, cre, cph]

rtpcr_2.1.9.tar.gz
rtpcr_2.1.9.zip(r-4.7)rtpcr_2.1.9.zip(r-4.6)rtpcr_2.1.9.zip(r-4.5)
rtpcr_2.1.9.tgz(r-4.6-any)rtpcr_2.1.9.tgz(r-4.5-any)
rtpcr_2.1.9.tar.gz(r-4.7-any)rtpcr_2.1.9.tar.gz(r-4.6-any)
rtpcr_2.1.9.tgz(r-4.6-emscripten)
manual.pdf |manual.html✨
DESCRIPTION |NEWS
card.svg |card.png
rtpcr/json (API)

# Install 'rtpcr' in R:
install.packages('rtpcr', repos = c('https://mirzaghaderi.r-universe.dev', 'https://cloud.r-project.org'))

Bug tracker:https://github.com/mirzaghaderi/rtpcr/issues

Pkgdown/docs site:https://mirzaghaderi.github.io

Datasets:

On CRAN:

Conda:

data-analysisqpcr

5.48 score 1 stars 20 scripts 703 downloads 11 exports 56 dependencies

Last updated from:8f491c0e99. Checks:7 NOTE, 2 OK. Indexed: yes.

TargetResultTimeFilesSyslog
linux-devel-x86_64NOTE176
source / vignettesOK186
linux-release-x86_64NOTE166
macos-release-arm64NOTE113
macos-oldrel-arm64NOTE104
windows-develNOTE141
windows-releaseNOTE105
windows-oldrelNOTE118
wasm-releaseOK152

Exports:ANOVA_DCtANOVA_DDCtcompute_wDCtefficiencylong_to_wideMeans_DDCtmeanTechmultiplotplotFactorTTEST_DDCtWILCOX_DDCt

Dependencies:bootclicodetoolscpp11dplyremmeansestimabilityfarvergenericsggplot2gluegtableisobandlabelinglatticelifecyclelme4lmerTestmagrittrMASSMatrixminqamultcompmultcompViewmvtnormnlmenloptrnumDerivpillarpkgconfigplyrpurrrR6rbibutilsRColorBrewerRcppRcppEigenRdpackreformulasreshape2rlangS7sandwichscalesstringistringrsurvivalTH.datatibbletidyrtidyselectutf8vctrsviridisLitewithrzoo

Calculation method
Calculations | A generalized calculation method | $$w\Delta C_t =\log_2(E_ | $$w\Delta C_t =\log_ | $$\text | References
Rendered frommethod.Rmdusingknitr::rmarkdown

Last update: 2026-06-19
Started: 2025-12-31

Manual
Overview | Running the shiny version of the rtpcr offline | Functions | Quick start | Installing and loading | Input data structure | NOTE 1 | NOTE 2 | NOTE 3 | NOTE 4 | Handling missing data | Data Analysis | Amplification Efficiency | Relative expression | NOTE | Examples | Output | Data output | Plot output | Plot output: example 1 | How to edit output plots? | Plot output: example 2 | Plot output: example 3 | Post-hoc analysis | Checking normality of residuals | Mean of technical replicates | Contact | Citation | References
Rendered fromManual.Rmdusingknitr::rmarkdown

Last update: 2026-06-19
Started: 2026-01-08